376 papers
This study demonstrates that APEX1 protects cardiomyocytes from oxidative stress-induced injury during ischemia-reperfusion by stabilizing p53 protein levels, identifying it as a promising therapeutic target for heart disease.
This paper introduces GRASP, a transformation-independent CRISPR-based method that utilizes dCas9-gRNA ribonucleoprotein complexes to directly capture and purify specific chromatin loci from native plant nuclei, enabling efficient, sequence-specific isolation of genomic regions and their associated components without the need for transgenic expression.
By analyzing fecal metagenomes from 1,034 individuals, this study constructs a comprehensive resource of gut bacteria, viruses, and plasmids to demonstrate that CRISPR-Cas immune repertoires primarily record local, cohort-specific interactions between bacteria and mobile genetic elements rather than reflecting direct human host characteristics.
This study introduces "in extracto" cryo-EM to achieve near-atomic resolution (~2.2 Å) of cellular lysates, revealing that elongation factor 2 (eEF2) is the predominant hibernation factor bound to both 80S and 60S ribosomes, alongside other regulatory proteins, thereby elucidating diverse native ribosome inactivation mechanisms.
This study demonstrates that ischemia-induced ubiquitination of the astrocytic glutamate transporter GLT-1 triggers its aberrant internalization and degradation, leading to impaired glutamate uptake, and shows that blocking this post-translational modification restores surface expression and provides neuroprotection.
This study demonstrates that dietary spermidine supplementation in farmed Atlantic salmon suppresses age-related adipose inflammation and restores metabolic flexibility by reprogramming lipid flux, while also establishing the species as a novel vertebrate model for studying metabolic ageing.
This study develops and validates a comprehensive molecular workflow comprising three highly sensitive real-time PCR assays and five Sanger primer sets to provide rapid, accurate, and verified species identification for the reemerging New World screwworm (*Cochliomyia hominivorax*), thereby enhancing surveillance capabilities for potential incursions into the United States.
This study identifies the chromatin-associated factor PHF13 as a critical regulator that maintains trophoblast stemness and restrains differentiation into syncytiotrophoblasts, thereby playing a vital role in placental development and preventing obstetrical complications.
By integrating neurogenic data with RNA-chromatin interaction mapping, this study reveals how noncoding RNA-transcription factor cooperation and human-specific regulatory rewiring, particularly involving TEAD2 and ONECUT2, shaped prefrontal cortex development and evolutionary divergence from extinct hominins.
This study evaluates the current state of mangrove biodiversity assessments in Kenya through a systematic literature review and a pilot environmental DNA (eDNA) survey, revealing significant spatial biases in existing data and demonstrating that eDNA offers a complementary approach to expand taxonomic detection, provided that regional reference databases and standardized frameworks are developed.
This study demonstrates that the RBM20-R636Q mutation drives atrial remodeling and arrhythmias through specific ion channel alterations, while SGLT inhibitors effectively mitigate these electrophysiological disturbances and reduce arrhythmogenesis.
This study utilizes spatial transcriptomics to demonstrate that TNBC lymph node metastases are characterized by expanded TNF/NF-κB-driven immune hubs centered on TMSB4X and CD74-expressing cells, which coordinate myeloid-endothelial crosstalk to promote metastatic persistence.
This study demonstrates that RNA Polymerase II acts as a central regulator of human chromatin packing domains by generating transcriptional loops that both assemble and maintain these nanoscale structures, thereby tightly coupling genome organization with RNA synthesis to optimize transcriptional responses.
By systematically relocating CTCF binding sites within the mouse Sox2 locus, this study reveals that these sites exert orientation-dependent effects on gene expression, either activating the promoter or repressing enhancer activity through mechanisms consistent with polarized loop extrusion.
This study utilizes bacterial hybrid assays and mutagenesis to characterize the conserved protein-protein interactions and species-specific RNA-binding mechanisms of the KH-domain proteins KhpA and KhpB across three human pathogens, revealing that GXXG motif variations dictate their distinct RNA recognition capabilities.
This study identifies digoxin as a specific inhibitor of Bax{Delta}2-induced neuronal cell death that acts independently of its cardiac effects by modulating protein levels rather than aggregation, suggesting that structurally similar, safer compounds could be developed to treat Alzheimer's disease.
This study identifies TXNDC5 as a critical driver of pulmonary hypertension by mediating hypoxia-induced extracellular matrix remodeling via biglycan in endothelial cells, thereby establishing it as a promising therapeutic target for the disease.
This study demonstrates that deanticoagulated heparin derivatives, specifically NALHP and its fragment S6, effectively treat ulcerative colitis by promoting goblet cell differentiation and mucosal barrier repair through the modulation of Wnt and Notch signaling pathways.
This study identifies circulating miR-10b-5p as a key driver of Kawasaki disease vasculitis that reprograms endothelial cells via the MKI67/CBX5-CEBPA axis to upregulate CXCL8, thereby establishing serum CXCL8 as a promising early diagnostic biomarker for the disease.
Using a rat model of MuSK myasthenia gravis, this study reveals that MuSK autoimmunity drives selective atrophy of slow-twitch muscle fibers through profound proteome remodeling characterized by disrupted mitochondrial and translational homeostasis, extending beyond mere neuromuscular junction impairment.